Responsibilities
· Assist in design and execution of cloning experiments, including plasmid construction and transformation.
· Execute studies for pre-CMC and CMC production by evaluating different microbial strains, optimizing fermentation processes, constructing plasmids, banking cells, and conducting microbial fermentations up to 10L scale.
· Gain proficiency in scaling up of processes from shake flasks to bioreactors.
· Support upstream activities which include but are not limited to basic cell culture, cell lysis, media and buffer preparation, and instrument cleaning / maintenance.
· Contribute to the writing of laboratory protocols, standard operating procedures, and other forms of routine documentation.
· Analyze experimental data, including gel electrophoresis and Nanopore sequencing data.
· Effectively communicate experimental data and their implications to internal stakeholders.
Requirements
· Bachelor’s or master's degree in Biological Science and Life Science disciplines, Biotechnology, Bioengineering or related field.
· At least one year of work experience in a bioprocessing, academic or commercial process development setting.
· Familiarity in engineering and culturing Escherichia Coli as host for plasmid production.
· Experienced in molecular biology techniques such as DNA extraction and purification, PCR, cloning and assembly, electrophoresis, and transformation.
· Demonstrate proficiency in designing and troubleshooting experiments.
· Work authorization in Singapore.